SOX2/STAT1/FOXO1 SIGNALING: NEW PATHWAY

What is SOX2? 

SOX2 is a transcription factor that is required for undifferentiated embryonic stem cells to retain their self-renewal or pluripotency. Sox2 is essential for the survival of embryonic and neural stem cells. Sox2 belongs to the Sox family of transcription factors, which have been found to play important roles in mammalian development at various stages. HMG (High-mobility group) box domains, which contain about 80 amino acids, are highly conserved DNA binding domains in this protein family.

Sox2 is required for somatic cell reprogramming, which involves returning differentiated cells to a pluripotent embryonic state by reversing their epigenetic arrangement. Sox2 is important for directing the differentiation of PSCs into neural progenitors and preserving the features of neural progenitor stem cells, in addition to its role in pluripotency control. 

About SOX2 antibody

SOX2 antibody is tested for specificity and affinity using WB, IHC, ICC, Immunofluorescence, and ELISA with known positive and negative samples. Immunizing mice with a synthetic peptide matching to amino acids surrounding Gly179 of human Sox2 produces SOX2 antibody. Protein A and peptide affinity chromatography are used to purify antibodies. Adult multipotent progenitors that give rise to some adult epithelial tissues, such as glands, the glandular stomach, testes, and cervix, also include Sox2. Sox2 is hypothesized to regulate target gene expression that is critical for these tissues’ survival and regeneration.

What is  STAT1 antibody? 

STAT1 (signal transducer and activator of transcription 1) is a transcription factor encoded by the STAT1 gene in humans. STAT1 antibody belongs to the STAT protein family. All STAT molecules are phosphorylated by receptor-associated kinases, resulting in activation, dimerization (creating homo- or heterodimers), and translocation to the nucleus, where they function as transcription factors. STAT1 is involved in the upregulation of genes in response to type I, type II, or type III interferon signals. STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma-Activated Sequence) promoter element in response to IFN gamma stimulation; STAT1 forms a heterodimer with STAT2 that binds to the ISRE (Interferon-Stimulated Response Element) promoter element in response to either IFN-alpha or IFN- beta stimulation.

Western Blot, Immunohistochemistry (Paraffin), Immunocytochemistry, Flow Cytometry, and Immunoprecipitation are just a few of the scientific applications for STAT1 antibodies. In human, mouse, rat, canine, and non-human primate tissues, these antibodies target STAT1. In Rabbit and Mouse, researchers have generated polyclonal, monoclonal, recombinant monoclonal, and recombinant polyclonal antibodies against STAT1. To validate specificity to STAT1, these antibodies were tested using cell treatment, relative expression, knockdown, knockout, and IP-MS. This targeted gene, also known as CANDF7, IMD31A, IMD31B, IMD31C, and signal transducer and activator of transcription 1-alpha/beta, encodes the protein signal transducer and activator of transcription 1′ in humans. The protein’s mass is stated to be 87.3 kilodaltons in structure. Canine, porcine, monkey, mouse, and rat orthologs are based on gene names.

What is FOXO1 antibody? 

FoxO1 Antibody detects FoxO1 and FoxO4 protein levels in the body (at 65-70 kDa). When FoxO1 (or FoxO4) is not phosphorylated, the antibody is sensitive to phosphorylation inside the antigenic region and will preferentially detect it. Animals are immunized with a synthetic peptide that matches the sequence of human FoxO1 to create a FoxO1 antibody. Protein A and peptide affinity chromatography are used to purify antibodies.

The Fox (forkhead box) family of transcription factors has a DNA binding region with a wing-like helix shape. The Fox (forkhead box) family has a subfamily called FoxO. There are four FoxO isoforms in humans. Active FoxO promotes the production of downstream genes in the FoxO signaling pathway, mediating a variety of cellular physiological activities. Many cellular physiological events, such as apoptosis, cell-cycle control, glucose metabolism, oxidative stress resistance, and lifespan, are influenced by the FoxO signaling pathway.